Description
This lab-intensive course focuses on the theoretical and practical applications of polymerase chain reaction (PCR), one of the most important tools of molecular biology, and has applications in a broad range of scientific disciplines, including clinical characterization and treatment of disease, forensic science, evolutionary biology, and genetics. The course exposes students to advanced PCR techniques including site-specific DNA mutagenesis, reverse transcriptase PCR to measure gene expression, High Resolution Melt Analysis (HRM) real time PCR to identify genetic diseases, and PCR Genotyping of criminal suspect DNA. This course is intended for students in their junior or senior year. Prerequisite: C- or better in BIO 231.