Schedule
Fri, 5:00 PM - 8:00 PM (1/20/2017 - 2/3/2017) Location: CCC SCI 137
Sat-Sun, 9:00 AM - 5:00 PM (1/20/2017 - 2/3/2017) Location: CCC SCI 137
Description
This laboratory-intensive weekend course covers the methodologies of protein purification, such as column chromatography and ammonium sulfate precipitation, quantification of protein concentration through colorimetric methods, and protein analysis through both denaturing and non-denaturing PAGE. Various methods of detection are used including Coomassie blue stain, an enzymatic assay, and Western blot. By the end of the course, students generate their own data, compare purification methods, and produce an end report in either a paper or poster format. This course is intended for students in their junior or senior year. Prerequisite: C- or better in BIO 231.